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Article

A simple in vivo assay for measuring the efficiency of gene length-dependent processes in yeast mRNA biogenesis.

The FEBS journal - 1 Feb 2006

Morillo-Huesca Macarena, Vanti Manuela, Chávez Sebastián

Abstract excerpt

We have developed a simple reporter assay useful for detection and analysis of mutations and agents influencing mRNA biogenesis in a gene length-dependent manner. We have shown that two transcription units sharing the same promoter, terminator and open reading frame, but differing in the length of their 3'-untranslated regions, are differentially influenced by mutations affecting factors that play a role in...

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PMID: 16441662
DOI: 10.1111/j.1742-4658.2005.05108.x

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