Article
V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment.
Proceedings of the National Academy of Sciences of the United States of America - 26 May 2009
MacCarthy Thomas, Kalis Susan L, Roa Sergio, Pham Phuong, Goodman Myron F, Scharff Matthew D, Bergman Aviv
Abstract excerpt
The somatic hypermutation of Ig variable regions requires the activity of activation-induced cytidine deaminase (AID) which has previously been shown to preferentially deaminate WRC (W = A/T, R = A/G) motif hot spots in in vivo and in vitro assays. We compared mutation profiles of in vitro assays for the 3' flanking intron of VhJ558-Jh4 region to previously reported in vivo profiles for the same region in the...
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