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Article

Diagnostic method validation: High resolution melting (HRM) of small amplicons genotyping for the most common variants in the MTHFR gene.

Clinical biochemistry - 1 Aug 2009

Norambuena Patricia A, Copeland Joshua A, Krenková Petra, Stambergová Alexandra, Macek Milan

Abstract excerpt

OBJECTIVES: According to OECD guidelines methods implemented in a diagnostic laboratory should be properly validated prior their implementation. For this purpose we selected genotyping by High Resolution Melting (HRM) of small amplicons using common variants in MTHFR as a model. DESIGN AND METHODS: We selected previously typed samples on which selected analytical validation-related parameters relevant to DNA...

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Source

Identifiers

PMID: 19427845
DOI: 10.1016/j.clinbiochem.2009.04.015

Genes

MTHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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