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Article

Single probe PCR melting curve analysis MTHFR C677T SNP sites.

Analytical biochemistry - 15 Apr 2021

Chen Kai-Yun, Xu Jiang-Xia, Wang Meng-Meng, Hu Die, Xie Fangfang, Huang Defa, Chen Jie, Yang Tong, Zhang Jiajian, Song Fangli, Huang Shao, Zhong Tianyu

Abstract excerpt

BACKGROUND: The detection and analysis of methylene tetrahydrofolate reductase (MTHFR) C677T single nucleotide polymorphism (SNP) from blood samples is time-consuming and costly. We aimed to establish a method to detect these SNPs by direct whole blood PCR and without DNA extraction. METHODS: Probes modified by different fluorescent groups on the same sequence were designed. Various MTHFR genotypes from direct...

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Share to Methylenetetrahydrofolate Reductase (NADPH2)
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Identifiers

PMID: 33450284
DOI: 10.1016/j.ab.2021.114102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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