Article
Probing the functional impact of sequence variation on p53-DNA interactions using a novel microsphere assay for protein-DNA binding with human cell extracts.
PLoS genetics - 1 May 2009
Noureddine Maher A, Menendez Daniel, Campbell Michelle R, Bandele Omari J, Horvath Monica M, Wang Xuting, Pittman Gary S, Chorley Brian N, Resnick Michael A, Bell Douglas A
Abstract excerpt
The p53 tumor suppressor regulates its target genes through sequence-specific binding to DNA response elements (REs). Although numerous p53 REs are established, the thousands more identified by bioinformatics are not easily subjected to comparative functional evaluation. To examine the relationship between RE sequence variation -- including polymorphisms -- and p53 binding, we have developed a multiplex format...
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