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Disulfide Bond Substitution by Directed Evolution in an Engineered Binding Protein

4 May 2009

Abstract excerpt

Breaking ties: The antitumour protein, neocarzinostatin (NCS), is one of the few drug-carrying proteins used in human therapeutics. However, the presence of disulfide bonds limits this protein's potential development for many applications. This study describes a generic directed-evolution approach starting from NCS-3.24 (shown in the figure complexed with two testosterone molecules) to engineer stable...

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PMID: 19415706
DOI: 10.1002/cbic.200800745

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