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Article

Stable heterodimers from remodeling the domain interface of a homodimer using a phage display library.

Journal of molecular biology - 4 Jul 1997

Atwell S, Ridgway J B, Wells J A, Carter P

Abstract excerpt

Structure-guided phage display was used to select for combinations of interface residues for antibody C(H)3 domains that promote the formation of stable heterodimers. A C(H)3 "knob" mutant was made by replacement of a small residue, threonine, with a larger one, tryptophan: T366W. A library of C(H)3 "hole" mutants was then created by randomizing residues 366, 368 and 407, which are in proximity to the knob on the...

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Identifiers

PMID: 9231898
DOI: 10.1006/jmbi.1997.1116

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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