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Disulfide-compatible phage-assisted continuous evolution in the periplasmic space

13 Oct 2021

Abstract excerpt

The directed evolution of antibodies has yielded important research tools and human therapeutics. The dependence of many antibodies on disulfide bonds for stability has limited the application of continuous evolution technologies to antibodies and other disulfide-containing proteins. Here we describe periplasmic phage-assisted continuous evolution (pPACE), a system for continuous evolution of protein-protein...

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PMID: 34645844
DOI: 10.1038/s41467-021-26279-8

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