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Article

Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv.

PloS one - 1 Jan 2017

Gąciarz Anna, Ruddock Lloyd W

Abstract excerpt

CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E. coli in the presence and absence of CyDisCo, with >90% being solubly expressed. Two scFv, those derived from natalizumab and trastuzumab, were solubly produced in high amounts even in...

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Identifiers

PMID: 29253024
DOI: 10.1371/journal.pone.0189964
PMCID: PMC5734687

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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