Article
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
Journal of immunological methods - 20 Mar 2008
Junutula Jagath R, Bhakta Sunil, Raab Helga, Ervin Karen E, Eigenbrot Charles, Vandlen Richard, Scheller Richard H, Lowman Henry B
Abstract excerpt
Cysteines with reactive thiol groups are attractive tools for site-specific labeling of proteins. Engineering a reactive cysteine residue into proteins with multiple disulfide bonds is often a challenging task as it may interfere with structural and functional properties of the protein. Here we developed a phage display-based biochemical assay, PHESELECTOR (Phage ELISA for Selection of Reactive Thiols) to rapidly...
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