Article
Genetic analysis of Xenopus transcription factor IIIA.
Journal of molecular biology - 18 Dec 1998
Bumbulis M J, Wroblewski G, McKean D, Setzer D R
Abstract excerpt
We describe a method for the genetic analysis of the DNA-binding properties of Xenopus transcription factor IIIA (TFIIIA). In this approach, a transcriptional activator with the DNA-binding specificity of Xenopus TFIIIA is expressed in yeast cells, where it specifically activates expression of a beta-galactosidase reporter gene containing one or more Xenopus 5 S rRNA genes that function as upstream activator...
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