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Article

Identification and detection of RNA-RNA interactions using the yeast RNA hybrid system.

Nature protocols - 1 Jan 2006

Piganeau Nicolas, Schroeder Renée

Abstract excerpt

To test RNA-RNA interactions in cells, we developed a yeast RNA hybrid system derived from the yeast three-hybrid system. In this setup, the activation of a reporter gene (HIS3 or lacZ) is dependent on the interaction of two RNAs. One ('RNA X') is fused to MS2 RNA, forming the bait, which binds to a fusion protein composed of the MS2 coat and the LexA proteins. The second ('RNA Y') is fused to an RNA-based...

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PMID: 17406297
DOI: 10.1038/nprot.2006.111

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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