Article
A rapid and simple electrophoretic method for the detection of mutations involving small insertion or deletion: application to beta-thalassemia.
Human genetics - 1 Oct 1991
Cai S P, Eng B, Kan Y W, Chui D H
Abstract excerpt
The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqueous 8% polyacrylamide gel. In each heterozygote with either a 2 to 25 bp deletion, but not with a 1 bp insertion,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.