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An improved method for the detection of genetic variations in DNA with denaturing gradient gel electrophoresis.

Mutation research - 1 Apr 1990

Takahashi N, Hiyama K, Kodaira M, Satoh C

Abstract excerpt

We have examined the feasibility of denaturing gradient gel electrophoresis (DGGE) of RNA:DNA duplexes to detect variations in genomic and cloned DNAs. The result has demonstrated that employment of RNA:DNA duplexes makes DGGE much more practical for screening a large number of samples than that...

Topics

DNA
Electrophoresis
Female
Gene Frequency
Genes
Genetic Techniques
Genetic Variation
Genotype
Globins
Humans
Male
Nucleic Acid Denaturation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA Probes

Restriction Mapping

Source

Identifiers

PMID: 2183038
DOI: 10.1016/0165-1161(90)90032-j

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2183038-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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