Article
Identification of the multiple beta-thalassemia mutations by denaturing gradient gel electrophoresis.
The Journal of clinical investigation - 1 Feb 1990
Cai S P, Kan Y W
Abstract excerpt
We used denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese population. By amplifying the beta-globin gene in four separate fragments and electrophoresing the amplified DNA in two gels, we were able to distinguish all the 12 known mutations on the basis...
Topics
- Base Sequence
- DNA
- Electrophoresis
- Heterozygote
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Thalassemia