Article
Detection of single base substitutions in total genomic DNA.
Nature - 1 Jan 2000
Myers R M, Lumelsky N, Lerman L S, Maniatis T
Abstract excerpt
Certain single base substitutions causing genetic diseases or resulting in polymorphisms linked to mutant alleles, alter a restriction enzyme cleavage site and can therefore be detected in total genomic DNA using DNA blots. Many base substitutions do not lead to an altered restriction site, but these can be detected using synthetic oligonucleotides as hybridization probes if the DNA sequence surrounding the base...
Topics
- Base Sequence
- DNA
- DNA, Recombinant
- Electrophoresis, Polyacrylamide Gel
- Globins
- Heterozygote
- Homozygote
- Humans
- Mutation
- Nucleic Acid Heteroduplexes
- Nucleic Acid Hybridization
- Thalassemia