Article
Detection of deletions, insertions and single nucleotide substitutions in cloned beta-globin genes and new polymorphic nucleotide substitutions in beta-globin genes in a Japanese population using ribonuclease cleavage at mismatches in RNA:DNA duplexes.
Mutation research - 1 Aug 1990
Hiyama K, Kodaira M, Satoh C
Abstract excerpt
The applicability of ribonuclease cleavage at mismatches in RNA:DNA duplexes (RNase cleavage method) for determining nucleotide variant rates has been examined in a Japanese population. DNA segments of various lengths obtained from 4 different regions of a normal and 3 thalassemic cloned human be...
Topics
- Base Composition
- Base Sequence
- Chromosome Deletion
- Cloning, Molecular
- DNA
- Female
- Genes
- Globins
- Humans
- Japan
- Male
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Pedigree
- Polymerase Chain Reaction
- Polymorphism, Genetic
- RNA Probes