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Article

Duplex PCR-RFLP for simultaneous detection of factor V Leiden and prothrombin G20210A.

Molecular and cellular probes - 1 Oct 2003

Lucotte G, Champenois T

Abstract excerpt

The authors have used a primer-engineered duplex polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) for the simultaneous detection of factor V Leiden and prothrombin G20210A mutations. The method involves the generation of HindIII RFLPs, and the restricted amplification products are analyzed by agarose gel electrophoresis in a single gel lane. This method is simple an inexpensive,...

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Identifiers

PMID: 14580402
DOI: 10.1016/j.mcp.2003.07.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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