Article
Combinatorial mutagenesis of three major groove-contacting residues of EcoRI: single and double amino acid replacements retaining methyltransferase-sensitive activities.
Gene - 30 Sept 1991
Osuna J, Flores H, Soberón X
Abstract excerpt
A library of mutant ecoRIR genes encoding EcoRI restriction endonuclease was generated using trinucleotide blocks and a combination of recombinant DNA procedures, including primer extension and the polymerase chain reaction. Codons corresponding to three amino acids (E144, R145 and R200), previou...
Topics
- Amino Acids
- Base Sequence
- Deoxyribonuclease EcoRI
- Escherichia coli
- Genes, Bacterial
- Genomic Library
- Methyltransferases
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Polymerase Chain Reaction
- Protein Engineering