Imaging DNA loops induced by restriction endonuclease EcoRII. A single amino acid substitution uncouples target recognition from cooperative DNA interaction and cleavage.

Abstract excerpt

EcoRII is a type IIE restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its DNA recognition site. Transmission electron microscopy provided direct evidence that EcoRII mediates loop formation of linear DNA containing two EcoRII recognition sites. Specific DNA binding of EcoRII revealed a symmetrical...

Topics

• Asparagine
• DNA
• DNA Footprinting
• DNA-Binding Proteins
• Deoxyribonuclease I
• Deoxyribonucleases, Type II Site-Specific
• Molecular Weight
• Mutagenesis, Site-Directed
• Mutation
• Nucleic Acid Conformation