Temperature-sensitive mutants of the EcoRI endonuclease.

doi:10.1006/jmbi.1997.1419

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Article

Temperature-sensitive mutants of the EcoRI endonuclease.

Journal of molecular biology - 19 Dec 1997

Muir R S, Flores H, Zinder N D, Model P, Soberon X, Heitman J

Abstract excerpt

The EcoRI endonuclease is an important recombinant DNA tool and a paradigm of sequence-specific DNA-protein interactions. We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105K, M157I, C218Y, A235E, M255I, T261I and L263F) and characterized activity...

Topics

Bacteriophage lambda
Crystallography, X-Ray
DNA Damage
DNA Mutational Analysis
DNA Repair
DNA, Bacterial
Deoxyribonuclease EcoRI
Escherichia coli
Models, Molecular
Mutation
Temperature

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