Article
Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants.
Gene - 30 Apr 1990
Oelgeschläger T, Geiger R, Rüter T, Alves J, Fliess A, Pingoud A
Abstract excerpt
We have developed an assay that allows analysis of the activity of EcoRI restriction endonuclease (ENase) and its mutants in vivo. This assay is based on the fact that wild type (wt) EcoRI ENase is toxic for Escherichia coli cells not expressing the EcoRI methyltransferase (MTase). The viability...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- Binding Sites
- DNA, Viral
- Deoxyribonuclease EcoRI
- Escherichia coli
- Genetic Vectors
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Site-Specific DNA-Methyltransferase (Adenine-Specific)
- Substrate Specificity