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Article

Asymmetric photocross-linking pattern of restriction endonuclease EcoRII to the DNA recognition sequence.

The Journal of biological chemistry - 19 Apr 2002

Mücke Merlind, Pingoud Vera, Grelle Gerlinde, Kraft Regine, Krüger Detlev H, Reuter Monika

Abstract excerpt

The EcoRII homodimer engages two of its recognition sequences (5'-CCWGG) simultaneously and is therefore a type IIE restriction endonuclease. To identify the amino acids of EcoRII that interact specifically with the recognition sequence, we photocross-linked EcoRII with oligonucleotide substrates that contained only one recognition sequence for EcoRII. In this recognition sequence, we substituted either...

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PMID: 11832480
DOI: 10.1074/jbc.M109311200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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