Article
Cloning, and molecular characterization of the GCV1 gene encoding the glycine cleavage T-protein from Saccharomyces cerevisiae.
Gene - 20 Feb 1997
McNeil J B, Zhang F, Taylor B V, Sinclair D A, Pearlman R E, Bognar A L
Abstract excerpt
We have isolated the gene encoding the glycine cleavage T-protein (GCV1) of the yeast Saccharomyces cerevisiae and shown through gene disruption and enzyme assays that inactivation of GCV1 destroys glycine cleavage function. A DNA fragment encoding the GCV1 gene was cloned by PCR amplification us...
Topics
- Amino Acid Oxidoreductases
- Amino Acid Sequence
- Aminomethyltransferase
- Base Sequence
- Carrier Proteins
- Cloning, Molecular
- Culture Media
- Fungal Proteins
- Mitochondria
- Mitochondrial Proteins
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- RNA, Messenger
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid