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Article

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.

Chemical communications (Cambridge, England) - 1 May 2018

Manuguerra Ilenia, Croce Stefano, El-Sagheer Afaf H, Krissanaprasit Abhichart, Brown Tom, Gothelf Kurt V, Manetto Antonio

Abstract excerpt

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

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Identifiers

PMID: 29662975
DOI: 10.1039/c8cc00738a
PMCID: PMC5944424

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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