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Article

Construction of stabilized proteins by combinatorial consensus mutagenesis.

Protein engineering, design & selection : PEDS - 1 Nov 2004

Amin N, Liu A D, Ramer S, Aehle W, Meijer D, Metin M, Wong S, Gualfetti P, Schellenberger V

Abstract excerpt

We constructed stabilized variants of beta-lactamase (BLA) from Enterobacter cloacae by combinatorial recruitment of consensus mutations. By aligning the sequences of 38 BLA homologs, we identified 29 positions where the E.cloacae gene differs from the consensus sequence of lactamases and constructed combinatorial libraries using mixtures of mutagenic oligonucleotides encompassing all 29 positions. Screening of...

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Identifiers

PMID: 15574484
DOI: 10.1093/protein/gzh091

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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