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A new DNA polymerase I from Geobacillus caldoxylosilyticus TK4: cloning, characterization, and mutational analysis of two aromatic residues.

Applied microbiology and biotechnology - 1 Aug 2009

Sandalli Cemal, Singh Kamalendra, Modak Mukund J, Ketkar Amit, Canakci Sabriye, Demir Ismail, Belduz Ali Osman

Abstract excerpt

DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identity). The gene was overexpressed in Escherichia coli and the purified enzyme was biochemically...

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PMID: 19365630
DOI: 10.1007/s00253-009-1962-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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