Article
Temperature dependence of accuracy of DNA polymerase I from Geobacillus anatolicus.
Biochimie - 1 Sept 2012
Cağlayan Melike, Bilgin Neş'e
Abstract excerpt
Klenow-like DNA polymerase I fragment from Geobacillus anatolicus (GF) was cloned and purified. The accuracy of GF was measured in vitro at three different temperatures under single turnover conditions as well as using a forward mutation assay. In pre-steady-state kinetic measurements, when temperature was raised from 22 °C to 50 °C, the rate (k(pol)) for cognate dTTP and non-cognate dATP nucleotide...
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