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Article

Novel zinc finger nuclease created by combining the Cys(2)His(2)- and His(4)-type zinc finger domains.

Bioorganic & medicinal chemistry letters - 15 May 2009

Negi Shigeru, Umeda Yoshiyuki, Masuyama Saeko, Kano Koji, Sugiura Yukio

Abstract excerpt

To improve the DNA hydrolytic activity of the zinc finger nuclease, we have created a new artificial zinc finger nuclease (ZWH4) by connecting two distinct zinc finger domains possessing different types of Zn(II) binding sites (Cys(2)His(2)- and His(4)-types). The overall fold of ZWH4 is similar to that of the wild-type Sp1 zinc finger (Sp1(zf123)) as revealed by circular dichroism spectroscopy. The gel mobility...

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Identifiers

PMID: 19359170
DOI: 10.1016/j.bmcl.2009.03.088

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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