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Article

Design of a colicin E7 based chimeric zinc-finger nuclease.

Journal of computer-aided molecular design - 1 Aug 2014

Németh Eszter, Schilli Gabriella K, Nagy Gábor, Hasenhindl Christoph, Gyurcsik Béla, Oostenbrink Chris

Abstract excerpt

Colicin E7 is a natural bacterial toxin. Its nuclease domain (NColE7) enters the target cell and kills it by digesting the nucleic acids. The HNH-motif as the catalytic centre of NColE7 at the C-terminus requires the positively charged N-terminal loop for the nuclease activity-offering opportunities for allosteric control in a NColE7-based artificial nuclease. Accordingly, four novel zinc finger nucleases were...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 24952471
DOI: 10.1007/s10822-014-9765-8
PMCID: PMC4104000

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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