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Article

Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo.

The EMBO journal - 1 Dec 1991

Johannes F J, Gallwitz D

Abstract excerpt

The yeast Saccharomyces cerevisiae has a single actin gene, ACT1, whose protein product is essential for cell viability. To study the structure-function relationship of this evolutionarily highly conserved protein, we have introduced into the gene several mutations leading to substitutions of amino acids that, by chemical crosslinking experiments, have previously been identified as potential sites for the...

Topics

Actins
Amino Acid Sequence
Blotting, Southern
Cross-Linking Reagents
DNA, Fungal
Fluorescent Antibody Technique
Genes, Fungal
Genes, Lethal
Molecular Sequence Data
Mutagenesis, Site-Directed
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Saccharomyces cerevisiae

Sequence Homology, Nucleic Acid

Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 1935913
DOI: 10.1002/j.1460-2075.1991.tb04965.x
PMCID: PMC453136

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1935913-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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