Article
Mapping actin surfaces required for functional interactions in vivo.
The Journal of cell biology - 1 Jul 1994
Holtzman D A, Wertman K F, Drubin D G
Abstract excerpt
An in vivo strategy to identify amino acids of actin required for functional interactions with actin-binding proteins was developed. This approach is based on the assumption that an actin mutation that specifically impairs the interaction with an actin-binding protein will cause a phenotype similar to a null mutation in the gene that encodes the actin-binding protein. 21 actin mutations were analyzed in budding...
Topics
- Actin Depolymerizing Factors
- Actins
- Binding Sites
- Carrier Proteins
- Cytoskeletal Proteins
- Fungal Proteins
- Genes, Fungal
- Genes, Lethal
- Membrane Glycoproteins
- Microfilament Proteins
- Models, Molecular