Article
A mutation in an ATP-binding loop of Saccharomyces cerevisiae actin (S14A) causes a temperature-sensitive phenotype in vivo and in vitro.
The Journal of biological chemistry - 12 May 1995
Chen X, Rubenstein P A
Abstract excerpt
The Ser14 hydroxyl group of actin is one of six groups that potentially form hydrogen bonds with the gamma-phosphate of the ATP bound in the cleft separating the two domains of the protein. To understand the importance of this group in actin function, we mutated Ser14 of Saccharomyces cerevisiae actin and studied the effects of these mutations in vivo and in vitro. Substitution of Cys of Gly resulted in cell...
Topics
- Actins
- Adenosine Triphosphate
- Alanine
- Base Sequence
- Binding Sites
- Genotype
- Kinetics
- Microscopy, Electron
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Myosins