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Article

Systematic mutational analysis of the yeast ACT1 gene.

Genetics - 1 Oct 1992

Wertman K F, Drubin D G, Botstein D

Abstract excerpt

We report the isolation and characterization of a synoptic set of site-directed mutations distributed throughout the single actin gene of Saccharomyces cerevisiae. Mutations were systematically targeted to the surface of the protein by identifying clusters of 2 or more charged residues in the primary sequence; every charged residue in a cluster was replaced with alanine. Mutations were recovered in high yield (34...

Topics

Actins
Alleles
Amino Acid Sequence
Base Sequence
DNA Mutational Analysis
DNA, Fungal
Diploidy
Genes, Fungal
Genes, Lethal
Introns
Models, Molecular
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Phenotype

Plasmids

Saccharomyces cerevisiae

Source

Open in PubMed Open full text

Identifiers

PMID: 1427032
DOI: 10.1093/genetics/132.2.337
PMCID: PMC1205140

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1427032-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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