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Article

Systematic mutational analysis of the yeast ACT1 gene.

Genetics - 1 Oct 1992

Wertman K F, Drubin D G, Botstein D

Abstract excerpt

We report the isolation and characterization of a synoptic set of site-directed mutations distributed throughout the single actin gene of Saccharomyces cerevisiae. Mutations were systematically targeted to the surface of the protein by identifying clusters of 2 or more charged residues in the primary sequence; every charged residue in a cluster was replaced with alanine. Mutations were recovered in high yield (34...

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PMID: 1427032
DOI: 10.1093/genetics/132.2.337
PMCID: PMC1205140

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