Article
A mammalian actin substitution in yeast actin (H372R) causes a suppressible mitochondria/vacuole phenotype.
The Journal of biological chemistry - 28 Oct 2005
McKane Melissa, Wen Kuo-Kuang, Boldogh Istvan R, Ramcharan Sharmilee, Pon Liza A, Rubenstein Peter A
Abstract excerpt
To determine the reason for the inviability of Saccharomyces cerevisiae with skeletal muscle actin, we introduced into yeast actin the first variant muscle residue from the C-terminal end, H372R. Arg is also found at this position in non-yeast nonmuscle actins. The substitution caused retarded growth on glucose and an inability to use glycerol as a sole carbon source. The mitochondria were clumped and had lost...
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