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Article

Development of an integrative DNA transformation system for the yeast Candida utilis.

FEMS microbiology letters - 15 Aug 1998

Rodríguez L, Chávez F P, Basabe L, Rivero T, Delgado J M

Abstract excerpt

We report here the development of an auxotrophic transformation system for the food yeast Candida utilis. To facilitate molecular studies in Candida utilis, we isolated auxotrophic strains for uracil biosynthesis by the combination of NTG-mutagenesis and 5-fluorotic acid (FOA) selection. The ura-mutation could be functionally complemented by the homologous URA3 gene. We used both, LiAc and electroporation methods...

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Identifiers

PMID: 9742706
DOI: 10.1111/j.1574-6968.1998.tb13166.x

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