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Article

Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase.

Protein engineering, design & selection : PEDS - 1 Apr 2004

Kursula I, Salin M, Sun J, Norledge B V, Haapalainen A M, Sampson N S, Wierenga R K

Abstract excerpt

The conformational switch from open to closed of the flexible loop 6 of triosephosphate isomerase (TIM) is essential for the catalytic properties of TIM. Using a directed evolution approach, active variants of chicken TIM with a mutated C-terminal hinge tripeptide of loop 6 have been generated (Sun,J. and Sampson,N.S., Biochemistry, 1999, 38, 11474-11481). In chicken TIM, the wild-type C-terminal hinge tripeptide...

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Identifiers

PMID: 15166315
DOI: 10.1093/protein/gzh048

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase.PMID 15166315Read ↗

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