Polyhedrin initiator codon altered to AUU yields unexpected fusion protein from a baculovirus vector.

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Article

Polyhedrin initiator codon altered to AUU yields unexpected fusion protein from a baculovirus vector.

BioTechniques - 1 Sept 1991

Beames B, Braunagel S, Summers M D, Lanford R E

Abstract excerpt

A recombinant baculovirus expression vector was constructed to express the core (capsid) protein of the hepatitis B virus. Along with the expected 21-kDa polypeptide, a second 24-kDa protein was observed. Immunoprecipitation and immunoblotting using a rabbit polyclonal anticore antiserum demonstrated that the two proteins were related. The core gene originally was cloned in-frame with the polyhedrin initiator...

Topics

Amino Acid Sequence
Animals
Baculoviridae
Base Sequence
Cells, Cultured
Codon
DNA, Viral
Genetic Vectors
Hepatitis B virus
Immunoblotting
Molecular Sequence Data
Moths

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Occlusion Body Matrix Proteins

Recombinant Fusion Proteins

Viral Core Proteins

Viral Proteins

Viral Structural Proteins

Source

Open in PubMed

Identifiers

PMID: 1931038

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1931038-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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