Article
Polyhedrin initiator codon altered to AUU yields unexpected fusion protein from a baculovirus vector.
BioTechniques - 1 Sept 1991
Beames B, Braunagel S, Summers M D, Lanford R E
Abstract excerpt
A recombinant baculovirus expression vector was constructed to express the core (capsid) protein of the hepatitis B virus. Along with the expected 21-kDa polypeptide, a second 24-kDa protein was observed. Immunoprecipitation and immunoblotting using a rabbit polyclonal anticore antiserum demonstrated that the two proteins were related. The core gene originally was cloned in-frame with the polyhedrin initiator...
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