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Article

A polylinker-derived sequence, PL, highly increased translation efficiency in Escherichia coli.

Journal of basic microbiology - 1 Jan 1999

Xu J, Mironova R, Ivanov I G, Abouhaidar M G

Abstract excerpt

Pokeweed (Phytolacca americana) antiviral protein (PAP) is a highly specific ribosome-inactivating glycosidase. The PAP gene was isolated and cloned in an expression vector containing a polylinker-derived sequence (PL) but devoid of a Shine-Dalgarno (SD) sequence. Surprisingly, E. coli cells transformed with this vector produced over twice the amount of PAP than that with the consensus SD sequence. Computer...

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Identifiers

PMID: 10071866
DOI: 10.1002/(sici)1521-4028(199903)39:1<51::aid-jobm51>3.0.co;2-0

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