Specific nucleotide substitutions in the burst sequence enhance polyhedrin expression in alphabaculoviruses: improvement of baculovirus expression vectors.

Abstract excerpt

Alphabaculoviruses produce a large number of occlusion bodies (OBs) in host cells during the late stage of infection. OBs are mainly composed of the viral product polyhedrin (POLH), and the extremely high-level transcription of the polh gene has been exploited to express foreign proteins in insect cultured cells, larvae, and pupae. This polh hyper-transcription requires the "burst sequence" located between the...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Baculoviridae
• Share to Bombyx