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An alternate proton acceptor for excited-state proton transfer in green fluorescent protein: rewiring GFP.

Journal of the American Chemical Society - 30 Jan 2008

Stoner-Ma Deborah, Jaye Andrew A, Ronayne Kate L, Nappa Jérôme, Meech Stephen R, Tonge Peter J

Abstract excerpt

The neutral form of the chromophore in wild-type green fluorescent protein (wtGFP) undergoes excited-state proton transfer (ESPT) upon excitation, resulting in characteristic green (508 nm) fluorescence. This ESPT reaction involves a proton relay from the phenol hydroxyl of the chromophore to the ionized side chain of E222, and results in formation of the anionic chromophore in a protein environment optimized for...

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PMID: 18179211
DOI: 10.1021/ja0754507
PMCID: PMC2552410

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