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Article

Photoswitching of E222Q GFP mutants: "concerted" mechanism of chromophore isomerization and protonation.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 28 Oct 2010

Abbruzzetti Stefania, Bizzarri Ranieri, Luin Stefano, Nifosì Riccardo, Storti Barbara, Viappiani Cristiano, Beltram Fabio

Abstract excerpt

Photochromic (i.e. reversibly photoswitchable) fluorescent proteins increasingly find applications as biomarkers for advanced bioimaging applications. From a mechanistic point of view, photochromicity usually stems from the reversible cis-trans photoisomerization of the chromophore. We demonstrated experimentally that cis-trans photoisomerization constitutes a very efficient deactivation pathway of isolated...

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PMID: 20859582
DOI: 10.1039/c0pp00189a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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