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Article

Protonation and conformational dynamics of GFP mutants by two-photon excitation fluorescence correlation spectroscopy.

The journal of physical chemistry. B - 24 Jul 2008

Bosisio C, Quercioli V, Collini M, D'Alfonso L, Baldini G, Bettati S, Campanini B, Raboni S, Chirico G

Abstract excerpt

GFP mutants are known to display fluorescence flickering, a process that occurs in a wide time range. Because serine 65, threonine 203, glutamate 222, and histidine 148 have been indicated as key residues in determining the GFP fluorescence photodynamics, we have focused here on the role of histidine 148 and glutamate 222 by studying the fluorescence dynamics of GFPmut2 (S65A, V68L, and S72A GFP) and its H148G...

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PMID: 18582099
DOI: 10.1021/jp801164n

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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