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Article

New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria.

BioTechniques - 1 Feb 2021

Mejia-Santana Adrian, Lloyd Cameron J, Klose Karl E

Abstract excerpt

New cloning vectors have been developed with features to enhance quick allelic exchange in gram-negative bacteria. The conditionally replicative R6K and transfer origins facilitate conjugation and chromosomal integration into a variety of bacterial species, whereas the sacB gene provides counterselection for allelic exchange. The vectors have incorporated the lacZ alpha fragment with an enhanced multicloning site...

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Identifiers

PMID: 33492170
DOI: 10.2144/btn-2020-0135

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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