Article
Tools for Rapid Genetic Engineering of Vibrio fischeri.
Applied and environmental microbiology - 15 Jul 2018
Visick Karen L, Hodge-Hanson Kelsey M, Tischler Alice H, Bennett Allison K, Mastrodomenico Vincent
Abstract excerpt
Vibrio fischeri is used as a model for a number of processes, including symbiosis, quorum sensing, bioluminescence, and biofilm formation. Many of these studies depend on generating deletion mutants and complementing them. Engineering such strains, however, is a time-consuming, multistep process that relies on cloning and subcloning. Here, we describe a set of tools that can be used to rapidly engineer deletions...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
