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Article

Engineering transcription factors with novel DNA-binding specificity using comparative genomics.

Nucleic acids research - 1 May 2009

Desai Tasha A, Rodionov Dmitry A, Gelfand Mikhail S, Alm Eric J, Rao Christopher V

Abstract excerpt

The transcriptional program for a gene consists of the promoter necessary for recruiting RNA polymerase along with neighboring operator sites that bind different activators and repressors. From a synthetic biology perspective, if the DNA-binding specificity of these proteins can be changed, then they can be used to reprogram gene expression in cells. While many experimental methods exist for generating such...

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Identifiers

PMID: 19264798
DOI: 10.1093/nar/gkp079
PMCID: PMC2677863

Genes

CRP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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