Article
cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.
The Journal of biological chemistry - 5 Jun 1990
Rydberg B, Spurr N, Karran P
Abstract excerpt
The O6-methylguanine-DNA methyltransferases are the most common form of cellular defense against the biological effects of O6-methylguanine in DNA. By screening a cDNA library with oligonucleotide probes derived from the active site amino acid sequence of the bovine methyltransferase, we have iso...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Chromosome Mapping
- Chromosomes, Human, Pair 10
- Cloning, Molecular
- DNA
- DNA Probes
- Deoxyribonuclease EcoRI
- Deoxyribonuclease HindIII
- Escherichia coli
- Gene Expression
- Humans
- Methyltransferases
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Nucleic Acid Hybridization