Article
SsoII-like DNA-methyltransferase Ecl18kI: interaction between regulatory and methylating functions.
Biochemistry. Biokhimiia - 1 Jan 2009
Fedotova E A, Protsenko A S, Zakharova M V, Lavrova N V, Alekseevsky A V, Oretskaya T S, Karyagina A S, Solonin A S, Kubareva E A
Abstract excerpt
The interaction of DNA-methyltransferase Ecl18kI (M.Ecl18kI) with a fragment of promoter region of restriction-modification system SsoII was studied. It is shown that dissociation constants of M.Ecl18kI and M.SsoII complexes with DNA ligand carrying a regulatory site previously characterized for M.SsoII have comparable values. A deletion derivative of M.Ecl18kI, Delta(72-379)Ecl18kI, representing the N-terminal...
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