Article
Protein engineering of the restriction endonuclease EcoRV: replacement of an amino acid residue in the DNA binding site leads to an altered selectivity towards unmodified and modified substrates.
Biochimica et biophysica acta - 13 Sept 1994
Wenz C, Selent U, Wende W, Jeltsch A, Wolfes H, Pingoud A
Abstract excerpt
According to the crystal structure analysis of a specific EcoRV/DNA complex, the thymine residues of the recognition sequence -GATATC- are not in direct contact with any amino acid residue of the protein. However, several amino acid residues are sufficiently close that it seemed worthwhile trying...
Topics
- Asparagine
- Base Sequence
- Binding Sites
- Circular Dichroism
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Denaturation
- Oligodeoxyribonucleotides