Article
Inhibition of the restriction endonuclease BanII using modified DNA substrates. Determination of phosphate residues critical for the formation of an active enzyme-DNA complex.
The Journal of biological chemistry - 25 Aug 1990
Olsen D B, Kotzorek G, Sayers J R, Eckstein F
Abstract excerpt
The restriction endonuclease BanII catalyzes the cleavage of double-stranded DNA and recognizes the degenerate sequence 5'-GPuGCPyC-3'. The poly-linker of M13mp18 contains one such sequence, 5'-GAGCTC-3'. The three other possible sites recognized by the enzyme were prepared by site-directed muta-...
Topics
- Base Sequence
- Binding Sites
- Coliphages
- DNA, Viral
- Deoxyribonucleases, Type II Site-Specific
- Hydrolysis
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Substrate Specificity